mejbcart on Steve Kirsch's newsletter

[mejbcart]

since nobody cares for my posts, for a long time, I'll post the recent VARIANTS analysis in one of the SCIENCE papers, here in comments, OK? Sorry for this long text, it is important though. That addition posted yesterday at: https://mejbcart.substack.com/p/whatif-ace2-scenario-and-the-hell at the end is the following:

https://onlinelibrary.wiley.com/doi/10.1002/jmv.27927 titled “Omicron (BA.1) and sub-variants (BA.1.1, BA.2, and BA.3) of SARS-CoV-2 spike infectivity and pathogenicity: A comparative sequence and structural-based computational assessment“ by Suresh Kumar et al. in Jun 2022. Now check it for yourself.

At https://www.rcsb.org/fasta/entry/7T9L/display is the sequence of the Spike and ACE2 used in this publication to predict the PHYSIOLOGICAL parameters, which will set people in isolation!!! Take ACE2 first and compare the sequence with NATURAL human ACE2 at https://www.uniprot.org/uniprotkb/Q9BYF1/entry. For ACE2:

- missing the entire N-terminal portion ‘MSSSSWLLLSLVAVTAA’ in the model

- the residue 616Q in natural ACE2 was changed to CHARGED His! but this was NOT enough!

- the ENTIRE C-terminal in MODELLED ACE2 is MISSING: SIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF ==»> ONE HUNDRED EIGHTY NINE residiues were ERAZED and instead s.c. Histidine tag attached: HHHHHHH , all POSITIVEY CHARGED!!!

For the ‘mutated’ Spike VARIANT:

- the entire C-terminal section of the wild type SPike is NOT there in the ‘model’ YIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDD SEPVLKGVKLHYT

- the furin site is MISSING, BLAST comparison of the model with the natural Spike:

Query 655 NSYECDIPIGAGICASYQTQTKSHGSASSVASQS VARIANT MODEL

NSYECDIPIGAGICASYQTQT S A SVASQS

Sbjct 658 NSYECDIPIGAGICASYQTQTNSPRRARSVASQS Natural Spike

the model has 1285 residues, the ‘natural’ 2020 PATENTED Spike only 1273 residues=> the VARIANTS are GROWING and loosing entire sections of their sequences???!!!! And now read the PUBLISHED abstract in THE MOST PRESTIDIOUS JOURNAL of all times ((2022) Science 375: 760-764), for that 3 dimensional VARIANT analysis at https://www.rcsb.org/structure/7t9l , quote:

“The newly reported Omicron variant is poised to replace Delta as the most prevalent SARS-CoV-2 variant across the world. Cryo-EM structural analysis of the Omicron variant spike protein in complex with human ACE2 reveals new salt bridges and hydrogen bonds formed by mutated residues R493, S496 and R498 in the RBD with ACE2. These interactions appear to compensate for other Omicron mutations such as K417N known to reduce ACE2 binding affinity, resulting in similar biochemical ACE2 binding affinities for Delta and Omicron variants. Neutralization assays show that pseudoviruses displaying the Omicron spike protein exhibit increased antibody evasion. The increase in antibody evasion, together with retention of strong interactions at the ACE2 interface, thus represent important molecular features that likely contribute to the rapid spread of the Omicron variant.“

Every single process here is a COMPUTER SIMULATION resulting in The Table 1. Its title: “The predicted effect of Spike protein single mutations of Omicron variants on pathogenicity by using PredictSNP tool.”

Table 2 titled “Docking analysis of single-point mutation of Wuhan-RBM (receptor-binding motif), Omicron (BA.1)-RBD, and sub-variants (BA1.1, BA.2, and BA.3)-RBD residues with ACE2 using HEX software.” lists docking energies WITHOUT units…

Table 3 is interesting, salt bridge formation and hydrogen bond formation between ACE2-RBD predicted for Omicron and sub-variants using PDBePISA (PISA) web-based tool.

Salt bridge formation(ACE2‐RBD) Hydrogen bondformation (ACE2‐RBD)

WTa ASP 30-Lys417 Gln24-Asn487 (PDB ID: 6MOJ) Asp30-Lys417

BA.1.1 GLU 57-LYS 478 LYS 31-TYR 450

GLU 35-ARG 490 LYS 68-TYR 470

GLU 35-ARG 493 GLN 42-ALA 481

ASP 38-ARG 490 GLN 42-CYS 485

ASP 30-ARG 495 THR 27-THR 497

ASP 30-ARG 498 ASP 30-TYR 446

BA.2 ASP 30-ARG 490 ARG 559-ALA 472

ASP 30-ARG 490 GLN 388-ASN 484

GLU 35-ARG 495 LYS 68-THR 497

ASP 38-HIS 502 ASP 30-ARG 490

ASP 38-ARG 400 GLU 35-ARG 495

As you can see there are basically FOUR NEGATIVELY charged amino acids on the ACE2 interface, Asp30, Asp38, Glu34, Glu57, to which the positively charged Spike mutants attach with the strongest electrostatic forces, all caused by the total charge of -4 on the FIXED ACE2 surface. No matter how many positively charged Spike residues are around, that binding will take place if there is no steric hindrance to it. And now the CUT off of the first N-terminal residues in the ACE2 molecule, even if uncharged, will leave ‘lot of space’ for possible ‘new variants’…

Last but not least, adding SINGLE positively charged AMINO ACIDS, L-Lys, L-Arg, L-His to your diet, will help to neutralize and shield the ‘however mutated’ spikes… There are some studies confirming exactly that:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264737/ →supplementation with zinc and zinc ionophores; vitamins C, D3 and E; and l-lysine during covis19

https://pubmed.ncbi.nlm.nih.gov/34372507/ →lysine supplementation

https://www.wholefoodsmagazine.com/articles/8667-lysine-reported-to-halt-coronaviruses-an-interview-with-bill-sardi

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619186/ →arginine supplementation

I can only add one, this is a CRIMINAL SCAM, in particular the published conclusion, quote:

“In this study, Omicron (BA.1) and sub-variants (BA.1.1, BA.2, and BA.3) are compared and investigated with WT using various computational tools. There are 11 shared common mutations G339D, S373P, S375F, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, and N501Y in RBD Omicron and sub-variants that may contribute significantly to changing the host spectrum of SARS-CoV-2 in immune evasion and potential transmission. The Omicron sub-variants (BA.1.1, BA.2 and BA.3) are likely more transmissible than omicron (BA.1) and Delta.“

[jramsix]

Thanks for sharing.

I like reading the facts in detail.