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Jikky's ModernaGate CTCCTCGGCGGGCACGTAG theory was debunked by McKernan, who said that Jikky had dumped his reputation on a bible codes interpretation of BLAST and he was running cover for Baric: twitter.com/Kevin_McKer…, twitter.com/search?f=li…. The insert at the S1/S2 junction is 12 nucleotides long but the sequence that matched the M…
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Jikky's ModernaGate CTCCTCGGCGGGCACGTAG theory was debunked by McKernan, who said that Jikky had dumped his reputation on a bible codes interpretation of BLAST and he was running cover for Baric: https://twitter.com/Kevin_McKernan/status/1484987210508255233, https://twitter.com/search?q=from%3Akevin_mckernan+to%3Ajikkykjj+&f=live. The insert at the S1/S2 junction is 12 nucleotides long but the sequence that matched the Moderna patent also includes the next 7 nucleotides after the insert.
There's 14 possible 19-base segments that contain the entire 12-base insert and 7 surrounding bases. There's 29,884 different 19-base segments of Wuhan-Hu-1, but 1,669 or about 6% of them had an exact match to the human reference genome build 38:
brew install bowtie2 seqkit
wget ftp://ftp.ccb.jhu.edu/pub/data/bowtie_indexes/GRCh38_no_alt.zip;unzip GRCh38_no_alt.zip
curl -s 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=nuccore&rettype=fasta&id=MN908947'>sars2.fa
seqkit seq -s sars2.fa|awk -F '' '{for(i=1;i<length-18;i++)print ">"i"\n"substr($0,i,19)}'>frag.fa
bowtie2 -p4 --no-unal -x GRCh38_noalt_as/GRCh38_noalt_as --score-min C,0,-1 -fU frag.fa>temp
Jikky's segment also has 19/19 identical bases with a bird sequence titled "Chaetura pelagica apoptogenic 1, mitochondrial (APOPT1), partial mRNA" (https://archive.is/6qUW3).
Jikky wrote: "In order for that sequence to have arisen in that virus, the virus which was manufactured with its HIV inserts, had to have had been infected into patented cell lines supplied by Moderna that had that unique sequence not seen in any other virus." (https://www.arkmedic.info/p/how-to-blast-your-way-to-the-truth) However it's possible for bioweaponeers to edit the genome as a text file and then synthesize the whole genome, so they don't have to find a cell line which contains the 12-base segment they want to insert, then infect viruses with that cell line a zillion times until the right piece of the cell line's genome happens to get inserted to the right spot by chance. In the comments of Jikky's Substack post, Bioanon who is a PhD virologist also said that "the 'accidental' nature you implied is less likely than purposeful construction" (https://www.arkmedic.info/p/how-to-blast-your-way-to-the-truth/comment/4545048).
Jikky's 19-base segment matches bases 23601-23619 of Wuhan-Hu-1, which codes for the PRRAR codons along with 2 bases before it and 2 bases after it. The 19-base segment matches the 12-base insertion in Wuhan-Hu-1 and the next 7 bases. However if we go along with Jikky's theory where SARS2 acquired the insert in a cell culture from the MSH3 gene, it's not clear if the 7-base flanking region after the insert would've helped to facilitate accidental recombination much better than a 6-base or 5-base flanking region. And if the last two bases from Jikky's segment are left out so the flanking region is only 5 bases long, then the 17-base segment has a perfect match on BLAST to all of these patent sequences published before 2020:
$ curl 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=$nuccore&rettype=xml&id=HC903376,HC915932,HC908933,HC905783,HB443296,GN095906,BD179519'>temp.xml
$ xmlstarlet fo -D temp.xml|xml sel -t -m //GBSeq -v GBSeq_accession-version -o \| -v GBSeq_definition -o \| -v GBSeq_create-date -o \| -v .//GBReference/GBReference_title -v .//GBReference/GBReference_journal -o \| -n|awk -F\| '$3!~/202/'|grep -iv moderna
HC903376.1|Sequence 13528 from Patent EP2194140|18-JUN-2010|Process for the production of fine chemicalsEP2194140-A2 13528 09-JUN-2010 Metanomics GmbH (DE)|
HC915932.1|Sequence 26085 from Patent EP2194140|18-JUN-2010|Process for the production of fine chemicalsEP2194140-A2 26085 09-JUN-2010 Metanomics GmbH (DE)|
HC908933.1|Sequence 19086 from Patent EP2194140|18-JUN-2010|Process for the production of fine chemicalsEP2194140-A2 19086 09-JUN-2010 Metanomics GmbH (DE)|
HC905783.1|Sequence 15936 from Patent EP2194140|18-JUN-2010|Process for the production of fine chemicalsEP2194140-A2 15936 09-JUN-2010 Metanomics GmbH (DE)|
HB443296.1|Sequence 19 from Patent WO2009077406|14-JUL-2009|Lipid metabolism proteins, combinations of lipid metabolism proteins and uses thereofWO2009077406-A1 19 25-JUN-2009 BASF Plant Science GmbH (DE)|
GN095906.1|Sequence 687 from Patent WO2009037279|16-APR-2009|Plants with increased yieldWO2009037279-A1 687 26-MAR-2009 BASF Plant Science GmbH (DE)|
BD179519.1|Highly thermophilic bacterium-derived protein and gene encoding it|15-MAY-2003|Highly thermophilic bacterium-derived protein and gene encoding itJP2002325574-A 10 12-NOV-2002 THE INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH|
To reproduce the table above, go here: https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn. Enter `CTCCTCGGCGGGCACGTAG` to the field at the top but delete the last two bases, switch the database to "Patent sequences(pat)", and click BLAST. Then select the text in the results up to the last result where query coverage and identity are both 100%, copy the text, and run the code above.
The Moderna patent includes a total of 33,915 sequences. It's an unusual practice to include such a large amount of genetic material in a single patent, so McKernan called it a "kitchen sink patent". You can download all sequences from here: https://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US09587003B2#sequenceInfo. Their average length is about 1500 bases and their total length is about 53 megabases:
wget 'https://seqdata.uspto.gov/?pageRequest=download&type=SEQ&DocID=US09587003B2&DownloadSeq=Download+All+Sequences#sequenceInfo'
unzip US09587003-20170307-SUPP.ZIP
awk '/LENGTH/{x+=$NF;n++}END{print x,x/n,n}' Psips/Data/03/US/2017/870/095/B2/sequence/US09587003-20170307-S00001.TXT
I think the patent might contain nearly all human protein-coding genes, because there's about 32,500 protein-coding genes and their average length is about 1,340 bases. When I removed the poly(A) tail from Wuhan-Hu-1 and I tried searching for all possible 19-base segments within the sequences in the Moderna patent, there were 3 other segments besides Jikky's segment which had a perfect match to the patent:
bowtie2-build --thread 4 moderna.fa{,}
seqkit seq -s sars2.fa|sed 's/A*$//'|awk -F '' '{for(i=1;i<length-18;i++)print ">"i"\n"substr($0,i,19)}'>frag.fa
sed 's/.*SEQUENCE: />/;/^[< ]/d;s/ *[0-9]*\r$//;s/ //g' Psips/Data/03/US/2017/870/095/B2/sequence/US09587003-20170307-S00001.TXT|seqkit grep -svrp '[^acgt]'>moderna.fa
(echo sars2_start_pos patent_sequence_number match_start_in_patent_sequence sars2_segment;grep -v ^@ moderna.sam|cut -f1,3,4,10)|column -t
The burden of proof is on you to prove existence of nucleotides if you still claim that they are real.