Good starting point, but not all NGS are the same. IonTorrent (TF) and Illumina are much lower fidelity than PacBio or ONT. Multiplatform sequencing is the best (you use ALL the tools, combining the best features of each and verifying them against each other):
Good starting point, but not all NGS are the same. IonTorrent (TF) and Illumina are much lower fidelity than PacBio or ONT. Multiplatform sequencing is the best (you use ALL the tools, combining the best features of each and verifying them against each other):
Is there any type of NGS sequencing that can read the entire genome (i.e. 30k+ base pairs) or are they all working on smaller fragments which are then assembled and/or aligned?
However, NOT a single published and peer-reviewed ONT/PacBio long single read sequence (without PCR) of Pfizer or Moderna Covid-19 "vaccine" mRNA sequence exist in the scientific literature. There is ONE (1) putative sample posted on Github done on Illumina. That's it.
Thanks for the reference. Unless there is another part of the paper I am not seeing, the paper states, "These reads did not cover the entire SARS-CoV-2 reference genome; however, these were sufficient for mapping the N gene."
I am looking for a paper that states they read the entire genome. Do you know of one?
Good starting point, but not all NGS are the same. IonTorrent (TF) and Illumina are much lower fidelity than PacBio or ONT. Multiplatform sequencing is the best (you use ALL the tools, combining the best features of each and verifying them against each other):
https://precision.fda.gov/challenges/10/results
Unfortunately c. 90% of all NGS sequencing worldwide is done solely on the Illumina's platform.
Is there any type of NGS sequencing that can read the entire genome (i.e. 30k+ base pairs) or are they all working on smaller fragments which are then assembled and/or aligned?
Both PacBio and ONT systems can do single long reads beyond 30kbp of RNA. PacBio's SMRT doesn't require PCR amplification for long reads.
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-1935-5
SARS-CoV-2 samples have been long read sequenced without PCR amplification using ONT Minion.
https://pubmed.ncbi.nlm.nih.gov/35054462/
However, NOT a single published and peer-reviewed ONT/PacBio long single read sequence (without PCR) of Pfizer or Moderna Covid-19 "vaccine" mRNA sequence exist in the scientific literature. There is ONE (1) putative sample posted on Github done on Illumina. That's it.
Thanks for the reference. Unless there is another part of the paper I am not seeing, the paper states, "These reads did not cover the entire SARS-CoV-2 reference genome; however, these were sufficient for mapping the N gene."
I am looking for a paper that states they read the entire genome. Do you know of one?